Welcome to RAISIN

Rabies-Assisted Interrogation of Synaptic Infralimbic Networks

This data resource contains the quantitative breakdown of afferent neurons that form synapses onto specific postsynaptic neurons within the mouse infralimbic cortex. We’ve produced this resource by combining rabies-based transsynaptic tracing strategies with either targeted Cre knockin mice or viral tools that permit genetic access to specific IL neurons. In the first strategy, transgenic targeting of Cre recombinase was driven by the promoter of genes expressed in subsets of cortical interneurons, including PV-Cre , SST-Cre , VIP-Cre , and NDNF-Cre. The second strategy used the viral tool RetroAAV-Cre to gain access to distinct IL projection neurons that target the BLA , LEC , RE , and PAG.

The contents of this resource include browsable serial coronal sections from these experiments, as well as a searchable database that displays the quantification of inputs to user-defined postsynaptic IL neurons; both are easily downloadable from the site. The full image data (including all replicates) will be available by request on account of its size (approximately 5 GB per brain: total of 130 GB).

We encourage interested parties to consult the bioRxiv (https://www.biorxiv.org/content/10.1101/2021.03.12.435140v1) or peer-reviewed published manuscript (link here) for more information regarding additional experimental data, control experiments, and our analyses. In addition, all raw data can be found on our figshare page. https://doi.org/10.6084/m9.figshare.13302731.